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Part:BBa_K1936001:Design

Designed by: Marcel Dickmanns   Group: iGEM16_Duesseldorf   (2016-10-13)


Tom5-eGFP-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 33
    Illegal BamHI site found at 712
    Illegal XhoI site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

Take a look at the main page for further information on the design of this part.

Source

All used sequences except for linkers were codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.

Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369

LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]

References

[1] Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.